研究意義:

細胞中，細胞色素P450主要分布在內質網和線粒體內膜上，作為一種末端加氧酶，參與了生物體內的甾醇類激素合成等過程。近年來，對細胞色素P450的結構、功能特別是對其在藥物代謝中的作用的研究有了較大的進展。研究表明細胞色素P450是藥物代謝過程中的關鍵酶，而且對細胞因子和體溫調節(jié)都有重要影響。

相關文獻:

【文獻標題】Enantioselective toxicity ａnd oxidative stress effects of acetochlor on earthworms (Eisenia fetida) by mediating the signaling pathway

【作者】Yalei Liu, Kuan Fang, Xiaolian Zhang,et.al

【作者單位】中國農業(yè)科學院煙草研究所（Tobacco Research Institute of Chinese Academy of Agricultural Sciences）

【文獻中引用產品】

蚯蚓8-羥基脫氧鳥苷（8-OHdG）ELISA試劑盒

蚯蚓細胞色素P450(CYP450)ELISA試劑盒

蚯蚓羥基自由基(OH)ELISA檢測試劑盒

【關鍵詞】Acetochlor,Enantiomer,Earthworm,Toxicity,Transcriptomic

【DOI】doi.org/10.1016/j.scitotenv.2020.142630

【影響因子(IF)】8.6

【出版期刊】《Science of the Total Environment》

【產品原文引用】

The OH content, 8-OhdG content, ａnd CYP450 content were determined according to the method of Zhang et al. (2020) using a kit obtained from the ShangHai HengYuan Biological Technology Co., Ltd. (Shanghai, China). The OH content, 8-OHdG content, ａnd activity of P450 in earthworms were detected by the double antibody sandwich method. The OH in earthworms combined with the antibodies of OH marked by HRP (Horseradish Peroxidase). Then, 3,3′,5,5′-tetramethylbenzidine (TMB) was added to produce a chromogenic reaction with the compound of antibody-antigen-antibody labeled by the enzyme. The absorbance of the reaction solution was recorded at 450 nm. The detection principle ａnd method of 8-OhdG ａnd CYP450 are the same as those of OH.

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